Technical Specification: Electrophoresis Reagents¶

Official Source

Technical documentation published by Beijing Solarbio Science & Technology Co., Ltd. For product procurement: solarbio.store | solarbio.store

1. Product Range¶

Product	SKU	Application
Agarose (Regular, Molecular Biology Grade)	A8201	Routine DNA/RNA separation, analytical gels
Agarose (Low Melting Point)	A8350	Preparative electrophoresis, gel extraction, in-gel enzymatic reactions
6× DNA Loading Buffer	D1010	Sample preparation for agarose gel loading
DNA Molecular Weight Markers (100 bp–15 kb)	M1100–M1700	Size estimation
50× TAE Buffer	T1060	Agarose gel electrophoresis running buffer
10× TBE Buffer	T1050	High-resolution agarose electrophoresis
GoldView Nucleic Acid Stain	G8140	Safe DNA staining for UV visualization
30% Acrylamide/Bis (29:1)	A1010	PAGE gel preparation

Product	Advantage
Regular Agarose (A8201)	Low EEO (≤0.13) minimizes DNA band distortion; DNase/RNase-free; consistent gel strength ≥1400 g/cm²
Low Melting Point Agarose (A8350)	Melts at ≤65°C — compatible with enzymatic reactions (ligation, restriction) at 37°C
DNA Markers (M1100–M1700)	Ready-to-load (already in 1× loading buffer); reference bands at 2× intensity for easy band identification
GoldView Stain (G8140)	Nonmutagenic alternative to EtBr; detectable under UV 254/365 nm; sensitivity comparable to EtBr
6× Loading Buffer (D1010)	Contains orange G (tracking dye) and xylene cyanol; includes glycerol for well sedimentation

Product	SKU	Fragment Range	Number of Bands	Reference Band	Concentration
DNA Marker I	M1100	100–600 bp	6	500 bp (×2 intensity)	200 ng/5 μL
DNA Marker II	M1200	100–1000 bp	8	500 bp (×2 intensity)	250 ng/5 μL
DNA Marker III	M1300	200–3000 bp	7	1500 bp (×2 intensity)	300 ng/5 μL
DNA Marker IV	M1400	100–3000 bp	9	1500 bp (×2 intensity)	350 ng/5 μL
1 kb DNA Ladder	M1500	250–10,000 bp	10	3000 bp (×2 intensity)	500 ng/5 μL
100 bp DNA Ladder	M1600	100–1500 bp	14	500 bp (×2 intensity)	400 ng/5 μL
λ DNA/HindIII Marker	M1700	125–23,130 bp	8	4361 bp	500 ng/5 μL

Parameter	Specification
Detection method	UV transillumination (254 nm or 302 nm) or blue light (480 nm)
Sensitivity (agarose gel)	1–3 ng dsDNA per band (comparable to EtBr)
Mutagenicity	Ames test negative (non-mutagenic)
Storage	RT (light-sensitive; store in dark bottle)
Recommended usage	5 μL per 50 mL gel (1× final)
Staining method	Pre-cast (add to molten agarose before pouring) or post-electrophoresis bath
Post-staining compatibility	Yes — soak gel in GoldView (1× in TAE/TBE) for 20–30 min

Gel %	Orange G (≈ 50 bp)	Bromophenol Blue (≈ 300 bp)	Xylene Cyanol FF (≈ 4 kb)
0.7% agarose	with front	600 bp	9 kb
1.0% agarose	with front	300 bp	4 kb
1.5% agarose	with front	150 bp	2 kb
2.0% agarose	with front	100 bp	1 kb

Parameter	1× TAE	1× TBE
Composition	40 mM Tris-acetate, 1 mM EDTA, pH 8.2	89 mM Tris-borate, 2 mM EDTA, pH 8.3
Buffering capacity	Lower (acetate)	Higher (borate)
Resolution of small fragments (100–500 bp)	Good	Excellent
Resolution of large fragments (> 5 kb)	Good	Good
DNA migration speed	Faster (higher current)	Slower (lower current)
Reusability	Typically single-use	2–3 times (discard if precipitates form)
Best for	Routine agarose gel electrophoresis, gel extraction	High-resolution electrophoresis, PAGE

Gel % (w/v)	Effective Separation Range	Best For	Voltage Recommendation
0.5%	1–15 kb	Large fragment separation, genomic DNA	1–3 V/cm (50–70 V for minigel)
0.7%	0.8–10 kb	Routine large fragment analysis	3–5 V/cm
1.0%	0.5–7 kb	Routine analysis	5–8 V/cm
1.2%	0.4–6 kb	Standard separation	5–8 V/cm
1.5%	0.2–3 kb	Small fragment analysis, PCR products	5–8 V/cm
2.0%	0.1–2 kb	SNP/indel detection, small PCR, RFLP	6–8 V/cm
3.0%	0.05–1 kb	Fine separation (use high-resolution agarose)	6–10 V/cm

Desired %	Agarose (g)	1× TAE (mL)	Melt time (min, MW)	Cool before pouring to
0.7%	0.35	50	1–2	55–60°C
1.0%	0.50	50	1–2	55–60°C
1.5%	0.75	50	2–3	55–60°C
2.0%	1.00	50	2–3	55–60°C

Desired %	30% Acrylamide/Bis (mL)	Water (mL)	10× TBE (mL)	Application
5%	2.5	6.0	1.0	Large dsDNA (> 500 bp)
8%	4.0	4.5	1.0	Standard dsDNA (200–500 bp)
12%	6.0	2.5	1.0	Small dsDNA (100–200 bp)
20%	10.0	0	1.0	Oligonucleotides, ssDNA

Issue	Cause	Solution
DNA bands smeared	DNA degraded	Check sample integrity; use fresh DNA
	Voltage too high	Reduce to 5 V/cm; excessive voltage generates heat which melts gel
	Too much DNA loaded	Reduce to 50–100 ng per band
	Gel too dilute	Use 1.2–1.5% for PCR products
DNA bands curved (smile effect)	Gel overheated	Reduce voltage; use TBE (better buffering); use thinner gel
	Uneven gel thickness	Ensure level casting surface
Faint or no bands	GoldView too dilute	Re-stain post-electrophoresis; check UV lamp
	DNA degraded	Check DNA integrity on test gel
	Loading buffer missing	Add 1× loading buffer; ensures sample sinks into wells
Primer-dimer or low MW bands visible	Expected — low MW species run with tracking dye	Set lower marker for primer-dimer detection; reduce PCR cycles
Well-defined but extra bands	Contamination	Use fresh buffer; clean gel casting tray and comb with 70% ethanol
Gel does not solidify	Agarose concentration too low	For < 0.7% gels, cool to 4°C before pouring; melt thoroughly
	Buffer concentration wrong	Use 1× TAE or 0.5× TBE
DNA runs backwards	Electrode polarity reversed	Swap red/black leads; DNA migrates toward positive (red) anode