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Certificate of Analysis Guide

COA Document Structure

Each Solarbio COA includes:

Section Content Example Values
Header Product name, SKU, lot number, manufacturing date lot: SR20240401, mfg: 2024-04-15
Specification Table Parameter, specification limit, test result, test method Appearance: white powder; Conforms
Physical Properties Appearance, color, physical state, solubility White crystalline powder; soluble in DMSO
Chemical Analysis Purity (HPLC/GC), pH, moisture, identity (NMR, MS) HPLC ≥99.0%; found 99.3%
Biological Testing Activity assay, endotoxin, sterility (if applicable) Endotoxin <0.5 EU/mL
Approval QA signature, release date, approval status Approved 2024-04-20

Test Parameters by Product Category

Product Category QC Tests Specification Limit Method
PCR master mixes Activity assay, DNase test, RNase test, sterility Amplification efficiency >90% of reference; Nuclease negative qPCR (GAPDH target); Fluorogenic substrate
DNA polymerases Specific activity, exonuclease, endonuclease 50–100 U/µL; Nuclease negative Radioactive substrate; Plasmid digestion
ELISA kits Calibrator recovery, precision, linearity Recovery 90–110%; CV <10% Four-parameter logistic curve fit
Antibodies Titer, specificity, concentration, purity Titer >512,000; >95% by SDS-PAGE ELISA; A₂₈₀; SDS-PAGE densitometry
Assay kits Standard curve R², blank OD, precision R² >0.99; Blank <0.10 OD; CV <5% Microplate reader
Small molecules Purity, identity, moisture, heavy metals ≥95–98%; NMR match; <0.5% H₂O; <10 ppm HPLC; ¹H NMR; KF; ICP-MS
Buffers pH, osmolality, sterility, endotoxin ±0.1; ±5%; Sterile; <0.5 EU/mL Meter; Osmometer; Filtration; LAL
Competent cells Transformation efficiency, contamination >10⁹ cfu/µg pUC19; No contamination Plate counting; 3-day sterility test

Sample COA Entry Format

----------------------------------------------------------------------
Certificate of Analysis
----------------------------------------------------------------------
Product: 2×Taq PCR Master Mix
SKU: PC1100                    Lot: PC20240501
MFG Date: 2024-05-10           EXP Date: 2026-05-09
----------------------------------------------------------------------
Parameter              Specification         Result
----------------------------------------------------------------------
Appearance             Clear, colorless      Conforms
                       viscous liquid
pH                     8.5 ± 0.5            8.7
Activity               Amplify 1 kb target  Cq = 18.2 ± 0.5
                       (50 ng gDNA)
DNase                  Not detectable       <1 pg/reaction
RNase                  Not detectable       <1 pg/reaction
Sterility              No growth (14 d)     No growth
Endotoxin              <0.5 EU/mL           0.12 EU/mL
----------------------------------------------------------------------
QA Approval: __________________
Date: 2024-05-15
----------------------------------------------------------------------

How to Request a COA

COAs can be requested via four methods:

  1. Solarbio Store — Order history page: COA available as PDF download for each purchased lot
  2. Customer service email: Send product SKU and lot number to technical support
  3. Product packaging — QR code: Scan the QR code on the label to access COA via mobile browser
  4. Bulk order COA: Available on request for wholesale and institutional accounts

Typical Response Time

Request Method Response Time
Store download Instant
QR code scan Instant
Email request Within 24 hours
Bulk order COA Within 48 hours

COA Verification

To verify the authenticity of any Solarbio COA:

  • Check the lot number format: YYYYMMDD + product code
  • Verify the COA QR code links to https://solarbio.store/coa/{lot-number}
  • Confirm QA approval signature and date
  • Compare physical product packaging lot number against COA lot number

Cross-References

solarbio.store